Safranine O Manufacturer & Supplier In India

Safranine O

Safranine O – High-Purity Biological Stain Manufacturer in India

Safranine O, also widely known as Basic Red 2, is one of the most extensively used cationic phenazinium dyes in the world. It is a premium-quality biological stain valued in histology, cytology, microbiology, Gram staining, cartilage and mucin staining, redox indication, and industrial dyeing applications. Identified by CAS Number 477-73-6 and Color Index CI 50240, Safranine O is known for its brilliant red staining performance, excellent solubility, and outstanding consistency across both laboratory and industrial use cases.
At GSP Dyes and Chemicals Pvt Ltd, we are recognized as one of India's most trusted Safranine O (Basic Red 2) manufacturers, suppliers, and exporters, delivering high-purity biological stains and phenazinium dyes to clients across India and over 30 countries worldwide. Backed by advanced manufacturing infrastructure, ISO-certified processes, and decades of expertise, we ensure unmatched product quality, dependable supply, and competitive global pricing.

ABOUT PRODUCT

What is Safranine O ?

Safranine O is a synthetic cationic dye belonging to the phenazinium class of basic dyes, chemically known as 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride. It appears as a dark green crystalline powder that produces a brilliant red solution when dissolved in water and ethanol. Recognized internationally as Basic Red 2, this dye is the standard counterstain in Gram staining procedures and a widely accepted certified biological stain used by laboratories around the globe.

CAS Number:477-73-6
TECHNICAL DATA

Key Specifications

Chemical Name 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride
Common Names Safranine O, Basic Red 2, Safranin O
CAS Number 477-73-6
pH (1% solution) 4.5 – 6.5
Loss on Drying ≤ 5%
Insolubles ≤ 1%
Color Index CI 50240
Molecular Formula C₂₀H₁₉ClN₄
Molecular Weight 350.85 g/mol
Appearance Dark Green Crystalline Powder
Solution Color Brilliant Red
Solubility Soluble in Water and Ethanol
Dye Content ≥ 98%
Maximum Absorbance (λmax) 530 – 534 nm
Class Phenazinium (Basic) Dye
INDUSTRIAL APPLICATIONS

Applications of Safranine O

Safranine O is the standard counterstain in Gram staining, used worldwide to color Gram-negative bacteria pink/red, allowing easy differentiation from Gram-positive bacteria stained purple by crystal violet.

Safranine O is widely used in plant histology to stain lignified tissues such as xylem, sclerenchyma, and cuticles bright red. It also stains chitin in fungi and is used in animal tissue staining.

Used in cytological preparations to differentiate cellular structures and identify abnormalities, making it valuable in clinical diagnostic and research labs.

Safranine O binds strongly to acidic proteoglycans, making it the gold-standard dye for Safranin O staining of cartilage, particularly used in osteoarthritis and connective tissue research.

Used in selective staining of mast cell granules for immunological and pathological investigations.

Safranine O serves as a reliable redox indicator in titrimetric and electrochemical analysis owing to its reversible color change.

Used as a leveling and brightening agent in electroplating baths, ensuring uniform metal deposition and superior surface finish.

Safranine O is also used to color silk, wool, leather, and paper, providing brilliant red shades with strong fastness properties.

Why Choose GSP Dyes and Chemicals Pvt Ltd for Safranine O?

GSP Dyes and Chemicals Pvt Ltd, headquartered in Bhayander, Maharashtra, is among India's most trusted Safranine O (Basic Red 2) manufacturers, suppliers, and exporters. We combine cutting-edge manufacturing technology, robust quality control, and exceptional customer service to deliver world-class biological stains globally.

ISO-Certified Manufacturing Facility

Our manufacturing operations comply with ISO 9001:2015 quality standards, ensuring superior quality and process consistency.

Industry-Leading Dye Content (≥98%)

We supply Safranine O with one of the highest dye contents available, ensuring exceptional staining brightness and reliability.

Bulk Production Capacity

With high-volume production capabilities, we cater to bulk orders, wholesale demands, and large industrial/research contracts at the most competitive global prices.

Global Export Network

We export Safranine O to USA, UK, Germany, China, Brazil, UAE, South Africa, Vietnam, Bangladesh, Indonesia, and Southeast Asia, supported by efficient logistics from JNPT and Mumbai port.

Direct-from-Manufacturer Pricing

Being a direct producer eliminates intermediaries, allowing us to offer the best Safranine O prices in India with no compromise on quality.

Custom Packaging Options

We offer flexible packaging — from 500 g lab packs to 25 kg industrial drums — based on customer requirements.

Comprehensive Technical Support

Our R&D team provides COA, MSDS, technical data sheets, and complete application support to help clients achieve optimal staining performance.

APPLICATION & SUPPLY

Industries We Serve

Histology & Histopathology Laboratories
Cytology & Diagnostic Centers
Microbiology & Gram Staining Labs
Hospitals & Pathology Centers
Pharmaceutical & Biotech Companies
Universities, Colleges & Research Institutions
Electroplating & Metal Finishing Industries
Surface Coating & Industrial Paint Manufacturers
Leather, Paper & Specialty Coloring Industries
Chemical Trading & Distribution Companies

Packaging & Shipping

We offer Phenosafranine in:

500 g / 1 kg HDPE jars
Laboratory Packs
5 kg / 10 kg containers
Research Bulk
25 kg fiber drums
Industrial Bulk
Custom packaging based on client requirements.
We ensure export-grade packaging with proper labeling, batch identification, COA, and MSDS documentation, allowing seamless customs clearance and on-time delivery via air, sea, and courier.

Safranine O Manufacturer in Maharashtra, India

GSP Dyes and Chemicals Pvt Ltd is a top-rated Safranine O (Basic Red 2) manufacturer in Mumbai, Thane, Bhayander, Boisar, and Tarapur, supplying to clients across Maharashtra, pan-India, and globally. Our strategic location near JNPT and Mumbai port ensures fast, reliable, and cost-effective international shipping.

Safety & Storage Guidelines

  • Store in a cool, dry, and well-ventilated area away from direct sunlight and heat.
  • Keep containers tightly sealed when not in use.
  • Avoid contact with strong oxidizing agents and incompatible chemicals.
  • Use protective gloves, goggles, and lab coats while handling.
  • Refer to the MSDS for complete safety information.
SUPPORT

Frequently Asked Questions (FAQs)

Safranine O is widely used in Gram staining (as a counterstain), histology, cytology, cartilage and mucin staining, mast cell granule staining, redox indication, and electroplating applications.

The CAS Number of Safranine O is 477-73-6, and its Color Index is CI 50240.

Yes, Safranine O and Basic Red 2 refer to the same compound and are used interchangeably across the industry.

Safranine O appears as a dark green crystalline powder that forms a brilliant red solution in water and ethanol.

We supply Safranine O with a minimum dye content of 98%, ensuring excellent staining performance and high purity.

Yes! GSP Dyes and Chemicals Pvt Ltd specializes in bulk and wholesale supply of Safranine O for industrial, research, and laboratory use.
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Order Premium Safranine O from India's Most Trusted Manufacturer

Whether you need Safranine O (Basic Red 2) for Gram staining, histology, cytology, cartilage and mucin staining, redox indication, electroplating, or industrial coloring, GSP Dyes and Chemicals Pvt Ltd is your one-stop destination for the highest-purity, ISO-certified, lab-grade and industrial-grade phenazinium dye in India.

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Get a free quote, technical data sheet, COA, MSDS, and exclusive bulk pricing. Place your bulk order today and discover why leading global brands, laboratories, hospitals, and research institutions trust GSP Dyes and Chemicals Pvt Ltd for premium dye solutions!

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